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Light microscopes use visible light sources including white light and lasers to image live and fixed samples. There are many different microscopy techniques including brightfield, phase contrast, differential interference contrast (DIC), fluorescence and confocal. The centre has a number of instruments covering these modes. Common techniques performed at the centre are listed below.
Confocal / Multiphoton Microscopy
| Andor Revolution Spinning Disk Confocal |
Olympus fluoview 300 |
Zeiss LSM 510 Confocal with tunable Ti-Sapphire femtosecond Laser | Witec Alpha 500 Raman Confocal Microscope | |||
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| Optigrid structured illumination microscope | Olympus Fluoview 1000 BRB | Inverted confocal time resolved system | ||||
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Widefield Microscopy
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Leica Teaching Microscopes |
Nikon Eclipse 400 | Metripol polarised light microscope | ||
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Nikon Eclipse 400 upright microscope |
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Live Cell Imaging
Imaging cells in real time can provide critical insights into the nature of biological cell and tissue function. At the centre we have three microscopes fitted with environmental chambers. These chambers can tightly control the temperature and humidity and CO2 levels so that living cells can be maintained in a healthy state during long term imaging experiments. The chambers are fixed and cover the whole microscope station. The Andor system is also fitted with a zero drift compensation (zdc) system to prevent focal drift during long term imaging.